Three years after surviving an attack by vampire Jerry Dandrige, Charley Brewster is now a college student in therapy, convinced that vampires aren’t real. He’s dating Alex Young, a psychology student, and the two visit his old friend Peter Vincent, who has returned to hosting Fright Night, his late-night horror show.
During the visit, Charley sees mysterious crates being delivered and encounters four strange individuals, one of whom—Regine Dandrige—immediately captivates him. That night, Charley dreams of Regine visiting and biting him. His therapist, Dr. Scott Harrison, assures him it’s just a side effect of trauma. Later, Charley spots his friend Richie Green entering Regine’s apartment and witnesses what appears to be Richie being drained of blood by Regine and her companion Belle. Horrified, he runs to Peter, and the two crash a party at Regine’s penthouse.
At the party, Richie appears unharmed, and Regine claims to be a performance artist. She seduces Charley with a hypnotic dance, while Peter notices that she casts no reflection—a telltale vampire trait. Outside the party, Regine reveals her true identity to Peter: she is Jerry Dandrige’s sister and has come for revenge. Peter flees, shaken.
That night, Regine visits Charley while he sleeps and bites him. He continues denying what’s happening, but starts to show signs of transformation—aversion to garlic and sunlight. After learning that Richie’s body has turned up, Charley finally accepts the truth. Meanwhile, Regine’s minions—Louie, Belle, and the bug-eating Bozworth—begin stalking Alex.
Peter tries to warn Charley and Alex, but they dismiss him. Peter Vincent is later arrested after attempting to kill Regine on live TV, who has taken over Fright Night. Alex and Charley are also arrested during a run-in with Louie. Dr. Harrison bails Alex out, only to reveal he, too, is a vampire. Alex kills him and breaks Peter Vincent out of the psychiatric hospital with the help of a sympathetic patient.
Alex and Peter race to Regine’s lair to rescue Charley, who was bailed out by Regine and is on the verge of becoming a vampire. Together, they defeat Regine’s vampire gang in a series of battles. Regine attempts to escape into her coffin, but finds it lined with Communion wafers. In the final confrontation, Peter Vincent exposes her to sunlight, destroying her.
In the aftermath, Charley and Alex joke about their experience, acknowledging that no one would believe what they’ve been through. As they embrace, the sound of a bat flying off suggests the vampire threat may not be over.
