Batman and Harley Quinn

A seasoned detective and strategist, Batman relentlessly pursues clues to stop Ivy and Floronic Man. He orchestrates interventions and uses tactical planning to outmaneuver the villains. While wary of Harley's rehabilitation, he recognizes the high stakes and leads the effort to protect Gotham and its allies.

Batman's former protégé, now a capable partner who tracks Harley and counters Ivy's faction. He is skilled in combat and grappling with personal loyalties, often placing himself at risk for the mission. His evolving relationship with Harley adds emotional tension that drives the plot forward.

Harley grapples with parole and a turbulent past as a psychiatrist while craving normalcy. She shows unpredictable yet pivotal resourcefulness, using emotional appeals and her psychiatric insight to influence others. Her alliance with Batman and Nightwing is tested, but her choices ultimately help avert catastrophe.

Ivy leads the eco-plot with Floronic Man, leveraging pheromonal control and ecological zeal. She demonstrates tenderness toward Harley yet remains driven by her environmental crusade. Her decisions hinge on balancing passion for the Green with the consequences of her plans.

A biotechnological villain seeking a planet-wide virus to convert humanity into plant hybrids. He champions radical ecological solutions and believes drastic transformation will heal the world. His scheming drives the central threat that the heroes must neutralize.

A kidnapped scientist with expertise in chemistry, botany, and biological warfare. His knowledge is instrumental to Ivy and Woodrue's plans, making him a key leverage point for both sides. His captivity underscores the ethical stakes of scientific exploitation.

A tough ARGUS operative who provides Batman with leads about Goldblum's disappearance. He represents authority and practical constraints in pursuing the case. His role grounds the mission in a sense of real-world stakes and logistics.

A guardian of the Green who appears to confront the ecological threat but chooses not to intervene directly. His presence signals the larger natural forces at play and the moral limits of intervention. He serves as a quiet beacon of ecological balance within the conflict.

A henchman within Ivy and Woodrue's network, encountered during a perilous escape sequence. His presence highlights the ensemble of villains without expanding the central menace. He embodies the operational layer of the antagonists' crime organization.