Batman: Gotham Knight consists of six distinct segments, each presenting unique tales that delve into the world of Gotham and its enigmatic hero.
The first chapter, Have I Got a Story for You, is penned by Josh Olson and animated by Studio 4°C. In this story, a street-savvy kid encounters his three friends at a skate park, each claiming they saw Batman earlier that day. The narrative unfolds in reverse order, offering three different perspectives on Batman's essence and abilities, mirroring the storytelling style of the classic 1950 film Rashomon. One child describes Batman as a shadowy figure, capable of vanishing and reappearing at will, akin to the Vampire Batman from the Batman & Dracula trilogy. Another portrays him as a half-human, half-bat entity, reminiscent of the Man-Bat. A third child sees him as a mechanical combatant capable of incredible feats, such as leaping over tall buildings. The excitement culminates when Batman confronts the Man in Black, leading to his capture at the skate park, aided by a fourth kid who, after witnessing Batman sustain injuries, perceives him as a human warrior in a costume rather than just a legend.
Moving onto Crossfire, a story written by Greg Rucka and animated by Production I.G, we follow Major Crimes Unit partners Crispus Allen and Anna Ramirez, selected by Lieutenant James Gordon for their mission. Their task involves transferring the recently apprehended Man in Black to Arkham Asylum—a former escapee known as Jacob Feely, notorious for his skills in electronics and explosives. While en route, the duo debates Batman's reliability, with Allen expressing skepticism, arguing he's merely an errand boy for a vigilante. Ramirez, however, defends Batman, noting the positive changes he has made in Gotham. Their exchange is cut short as they become ensnared in a fierce gang shootout involving the Russian Mob and Sal Maroni. In a critical moment, while Maroni threatens Ramirez, Batman comes to the rescue, subduing the criminals and disappearing as Allen and Ramirez reflect on the true nature of their ally.
The third segment, Field Test, features a narrative by Jordan Goldberg and is animated by Bee Train. Here, an accident involving a WayneCom satellite inspires Lucius Fox to devise an innovative device designed to deflect gunfire using electromagnetic technology. As Bruce Wayne attends a charitable golf event hosted by land developer Ronald Marshall, he secretly acquires Marshall's PDA, leading to an investigation into the mysterious death of activist Teresa Williams. Later that night, after transforming into Batman, he commandeers a boat to thwart both Maroni's and the Russian Mob's operations. Batman endeavors to establish peace between the gang leaders, but chaos ensues when a bullet ricochets, leading to an injured mobster being taken to the hospital. Batman reflects on the device's effectiveness, admitting, > "It works too well: I'm willing to put my life on the line to do what I have to. But it has to be mine, no one else's."
In Darkness Dwells, the next narrative written by David S. Goyer and animated by Madhouse, takes a darker turn. The police respond to a riot instigated during a sermon led by Cardinal O'Fallon. With Lieutenant Gordon, Crispus Allen, and Anna Ramirez involved in the investigation, they explore the ominous underground crypts where O'Fallon may have been taken by a monstrous figure. Batman analyzes the scene alongside Gordon, suspecting the Scarecrow's fear toxin is responsible. Upon descending into the depths, Batman encounters Killer Croc, emerging under the influence of the toxin himself. After a brutal showdown, Batman rescues O'Fallon from a death trial orchestrated by the Scarecrow, creating an explosion that allows for their escape.
The penultimate segment, Working Through Pain, showcases Batman injured as he is shot while trying to evade a hallucinating gang member, likely affected by the Scarecrow's toxin. Severely wounded, Batman recalls life lessons learned from a woman named Cassandra, who taught him the art of managing pain through various hardships. As he reflects on his resilience, a scene occurs where he comes to Cassandra's aid during a confrontation with some young aggressors, demonstrating his martial arts prowess, ultimately affirming his learned abilities. However, when Batman is trapped in a gutter filled with firearms, he realizes his struggle is far from over.
The concluding segment, Deadshot, written by Alan Burnett and animated by Madhouse, reveals Bruce Wayne grappling with memories of his parents' murder as he examines the guns retrieved from the gutter. Receiving word of a new assassin named Deadshot, who has been commissioned to take out Lieutenant Gordon, Batman positions himself as a protective guardian. The narrative escalates with Deadshot attempting to take out Gordon from a moving train, but Batman intervenes, only to find himself the primary target. After a tense confrontation where Batman falls off the train, he ultimately ambushes Deadshot, asserting his dominance. The story culminates with Bruce contemplating his life's fight against crime, ending with profound words: > "I've been trying to stop those two bullets all my life." The film wraps on a hopeful tone, with Bruce gazing at the Bat-Signal, reaffirming his role in Gotham's shadowy landscape.