Anacondas: Trail of Blood

A rugged trekker and key member of the expedition who withstands the Carpathian dangers, including the brutal attack by the anaconda. He endures the loss of an arm before ultimately being killed by one of Murdoch's mercenaries. His loyalty and persistence highlight the relentless human drive to survive in hostile territory.

A gruff, protective leader among the survivors who coordinates the group's response to the serpent and the mounting threats. He forms a tense alliance with Heather and Wendy while navigating betrayals and combat. He makes decisive moves to safeguard the others, even at personal risk.

A determined ally of Jackson who fights to survive the ordeal and supports the group's efforts to destroy the serum's misuse. She adapts quickly to danger, helps coordinate the defense, and contributes to the plan to end the threat.

Heather’s best friend, bold but ultimately undone by the pursuit and violence surrounding the serum. She tries to escape but is cut down during the chaos, underscoring the film’s brutal stakes.

A billionaire antagonist who bankrolls the serum project and deploys mercenaries to seize the device. His ambition to cure his bone cancer drives him to dangerous, unethical ends, ultimately meeting his demise at the hands of the evolving predator.

Dr. Amanda Hayes is the surviving expert on the snake attack and the expedition’s moral compass. She strategizes with Alex to locate the serum and takes decisive actions to destroy the orchids’ habitat, balancing science with ethical restraint.

Murdoch’s ruthless assassin who fights for the serum and relentlessly pursues his quarry. He embodies the film’s mercenary threat, meeting his end in the explosive confrontation during the chase.

An officer who serves as Murdoch’s rival in the serum race. He is scheming and pragmatic, navigating loyalties and money with perilous consequences.

Mercenary who participates in the pursuit and clashes with the survivors. He is knocked aside by Jackson and ultimately eliminated by Amanda in the final confrontation.

A trekker and paleopathology-focused ally who assists Amanda after being rescued. He contributes to the search for the serum and escapes danger with the others.