Tom and Jerry Cowboy Up!

A clever, resourceful cat who initially struggles to impress Betty but becomes an ally to Jerry and the ranch. He navigates a series of comedic mishaps, including tying himself up in attempts to perform tricks and getting tangled in cattle-related chaos. By joining forces with Jerry and the mice, he helps defend the ranch from Critchley’s scheme and contributes to fixing up the property.

A quick-witted, daring mouse who turns obstacles into opportunities. He outsmarts Tom at first but ultimately allies with him to protect the Double B Ranch. Along with his nephews, Tuffy, Scruffy, and Duffy, Jerry orchestrates clever plans to counter Clem and Critchley.

The owner of The Double B Ranch and sister to Bentley, she calmly manages the ranch and awaits her brother’s return. Betty embodies practicality and warmth, keeping the ranch running even as threats loom. Her presence motivates Tom and the others to protect their home.

Betty’s brother and a railroad clerk who has returned to the family site for a break from city life. He brings a mix of urban stories and a surprising fear of mice, which adds humor and vulnerability to the ranch dynamic. His arrival helps catalyze the plot as the family unites to defend their land.

The antagonist aiming to expand his control by taking over the ranch. He enlists Clem and uses a scheme involving prairie dogs digging tunnels and scarecrows to frighten livestock and lower the land’s value. Critchley’s plans are the central threat that unites Tom, Jerry, and Betty in defense of the ranch.

Critchley’s henchman who harbors hatred for prairie dogs and pursues the destabilization of the ranch. His actions contribute to the escalating tension and comic peril around the tunnels and distractive ploys. He ultimately faces the consequences of the community’s resistance.

One of Jerry’s nephews, known for an appetite that disrupts the family meal and adds to the film’s comic chaos. The trio of nephews—Tuffy, Scruffy, and Duffy—provide playful energy and moments of mischief around the ranch.

Another of Jerry’s nephews, contributing to the family’s lively dynamic and the film’s lighthearted mischief. Scruffy’s antics accompany the mice as they navigate the ranch’s challenges and help out in playful ways.

Jerry’s nephew who, along with his siblings, adds to the ensemble’s humor and misadventures. Duffy participates in the antics around the corral and the ranch’s improvised defenses.

A fellow traveler in the landscape who, along with a grayish she-cat, crosses paths with Tom and the mice. Bumpy helps populate the ranch’s world with additional character moments and adds to the film’s frontier atmosphere.