Ben Hildebrand (Mark Harelik) and Eric Kirby (Trevor Morgan) embark on a parasailing adventure above the mesmerizing waters surrounding Isla Sorna. However, their journey quickly turns dire when the boat's crew mysteriously vanishes, prompting Ben to make a split-second decision to detach the line before the boat crashes into the treacherous waters, leaving him and Eric adrift towards the ominous island.
Fast forward eight weeks, and the renowned paleontologist Dr. Alan Grant (Sam Neill) finds himself grappling with a lack of funding for his groundbreaking research on the intelligence of Velociraptors. During a conversation with his trusted colleague Ellie (Laura Dern), Grant excitedly shares his significant discovery of a resonating larynx found in fossilized raptor remains. Drawing from both this finding and his past harrowing experiences at Jurassic Park, he theorizes that the original Velociraptors exhibited remarkable social behavior. He postulates a fascinating idea: had they survived and continued to evolve, they could have easily asserted themselves as the dominant species on Earth instead of humans. Meanwhile, Grant’s assistant, Billy Brennan (Alessandro Nivola), utilizes cutting-edge 3D printing technology to create a replica of the Velociraptor larynx.
Enter Paul (William H. Macy) and Amanda Kirby (Téa Leoni), who approach Grant under the guise of a wealthy couple interested in funding his research—albeit with a rather unconventional condition: an aerial tour of Isla Sorna. Despite his reservations, Grant finds himself compelled to agree, joining Paul, Amanda, Billy, and the Kirbys' decidedly less reputable mercenary associates—Udesky (Michael Jeter), Cooper (John Diehl), and Nash (Bruce A. Young)—on a flight toward the island. However, during the journey, Grant uncovers the unsettling truth: the Kirbys have ulterior motives that involve landing on the island. When he voices his objections, Cooper takes matters into his own hands, knocking Grant unconscious.
Upon regaining consciousness, Grant is horrified to realize they have indeed landed. The group is soon confronted by a ferocious Spinosaurus that forces them to scramble back to the plane, abandoning Cooper, who tragically succumbs to the dinosaur's hunger. The situation spirals further out of control as the plane crashes into the dense forest. The survivors narrowly evade the Spinosaurus, but not before it destroys their only means of escape, devouring Nash in the process, who had the Kirbys' satellite phone. As they flee, the group faces yet another threat—a formidable Tyrannosaurus Rex. A fierce battle ensues between the two predators, resulting in the Spinosaurus emerging victorious.
As tensions mount, Grant discovers that Paul and Amanda are, in fact, a desperate divorced couple searching for their missing son, Eric, along with Amanda’s boyfriend, Ben. Their plea for assistance came after governmental agencies denied their requests for help, which led them to seek Grant’s expertise due to his previous encounter with dinosaurs on Isla Nublar. To their shared dismay, Grant reveals that his prior experience does not extend to Isla Sorna, leaving them all equally unprepared.
Through a series of harrowing encounters, they discover Ben's para-sail along with his lifeless body, and later stumble upon raptor nests, landing them in increasingly dangerous situations. After a series of thrilling escapades, including a stampede caused by fleeing raptors, the group is separated. Udesky suffers a grim fate when the cunning raptors trap him. In a stroke of luck, Eric, who has managed to survive in an overturned supply truck, assists Grant just in time.
The next day, after reuniting with Billy and the Kirbys, they find themselves once again pursued by the relentless Spinosaurus, and seek refuge in a deserted observatory. Unbeknownst to the group, Billy has taken two raptor eggs for personal gain, igniting a chain reaction of raptor attacks. To save the group, Grant takes the eggs for leverage and unwittingly leads them into a massive aviary filled with Pteranodons, where disaster strikes when Eric is carried off.
After a thrilling escape, during which Billy tries to save Eric but seemingly meets his own demise, the group manages to secure a boat and heads down a river. That night, a pivotal moment occurs when they retrieve the ringing satellite phone from the Spinosaurus’s feces, allowing Grant to contact Ellie for a rescue. A fierce encounter with the Spinosaurus ensues, leading Grant to ignite the boat's fuel, forcing the creature to flee.
As dawn approaches, they make their way to the coast, only to find themselves cornered by raptors. In a tense standoff, they surrender the eggs to the pack while Grant cleverly uses the replica raptor larynx to confuse them, allowing the group to escape. When they finally reach the coast, it is revealed that Ellie has successfully called in the Marine Corps and Navy for their rescue. Amidst the chaos, they discover that Billy, although injured, has also been rescued, and as they make their exit, they witness Pteranodons soaring overhead, marking the end of their treacherous adventure.
