Karen Christie Wagner leaves her mother's house after a visit and heads to a nearby garage, where a quick swap changes her ride from a car to a motorcycle. She also sheds her sun dress for street clothes—a blue jeans outfit with a green vest bearing a pink cat head and the word MAN-EATERS on the back. Reuniting with the close-knit circle she keeps secret from her mother, she reveals she is part of an all-female motorcycle gang known as the Man-Eaters. The crew hangs out in an abandoned house and hosts weekly motorcycle races on an old airport runway, with the winner earning first pick from the infamous “stud line.”
Queen Betty Connell, the tough-minded leader, runs the races and enforces a strict code: no Man-Eater is allowed to be tied to anyone or anything but the gang, and a brutal rule that “all men are mothers” forbids romantic attachment. During a race, Karen takes the win while Queen finishes second. Despite the rivalry, Queen proves to be a sportsman and lets Karen choose first. That day, Karen selects a familiar companion named Bill David Harris, a friendly guy she has picked for four weeks in a row. But the code is relentless, and the act of forming attachments is prohibited, which becomes clear when Honey Pot, the teenage mascot who longs to join the gang, is watching closely.
Honey Pot, believed to be on the verge of membership, reports Karen’s growing attraction to Bill to Queen. After much deliberation, Queen instructs Honey Pot to summon Karen to the runway that evening. When Karen arrives, the gang makes a startling demand: she must participate in a brutal punishment—dragging Bill, who is tied and beaten, along the length of the runway and back. The act ends with Bill’s death, and Karen’s loyalty to the Man-Eaters is sealed.
Honey Pot moves further up the ranks with a baptism of blood and ritual. In a hazing ceremony, her left hand is cut open for a blood oath, and she is made to kiss all the members to prove sisterhood. She is then stripped to her underwear, doused in honey and chocolate syrup, and paraded to entertain the entire “stud line.” Afterward, she is cleaned up, given a leather jacket with the Man-Eaters emblem, and formally inducted.
The next day, the Man-Eaters ride into the small Florida town of Medley, where they terrorize locals by tearing through parking lots, storefronts, and the yards of houses. The local police find themselves hamstrung, unable to file charges as fear of retribution keeps the townspeople silent. The gang leaves behind only a warning to stay out of trouble.
A week later, the stud line competition resumes, and the Man-Eaters head to the runway. An all-male gang has gathered along the sidelines, turning the playful race into a tense confrontation. Queen and the rival leader, Joe Boy John Weymer, clash with knives, triggering a fierce brawl between the two groups. The Man-Eaters emerge victorious, but the conflict echoes through Medley.
Ted [Rodney Bedell], a former boyfriend of Karen, tracks her down and pleads for her to leave the gang and return to ordinary life. Karen hesitates, torn between love and loyalty, telling him to forget about her. Ted, not ready to give up, infiltrates the stud line by disguising himself as one of the ruffians. After the race, Queen asserts her control by choosing another partner, but Karen recognizes Ted and chooses him for a private conversation. In the meantime, an orgy unfolds in the main living area with the Man-Eaters and their male followers. Ted warns Karen about Joe Boy and his gang’s intent on revenge after their humiliating defeat.
Honey Pot takes a breath of fresh air outside and is ambushed by the male gang. The next morning, Honey Pot is returned to the Man-Eaters battered and bear a brutal nose ring, the wounds fresh and severe. Joe Boy’s gang leaves a grim note threatening further retribution if there is more trouble. The group transports Honey Pot to a hospital and then sets out to find Joe Boy and his rivals.
A bar visit yields little information, and the bartender suffers a brutal beating at the hands of Queen. Two members, Whitey Pat Poston and Terry Ruby Tuesday, spot Joe Boy and his gang at a local restaurant, reporting back to Queen. An ambush is planned: Whitey and Terry return to the restaurant to slash Joe Boy’s car tires, and when Joe Boy confronts them, Whitey sprays bug spray in his face before retreating on a motorcycle. Joe Boy gives chase, triggering Queen’s carefully laid trap along a secluded stretch of road.
Lying in wait with Karen, Supergirl, Ginger, Delta, and the rest of the Man-Eaters, Queen uses a nearly invisible wire to snag Joe Boy as he pursues the decoy riders. Whitey and Terry lure Joe Boy toward the trap, and he is decapitated as he collides with the wire, his severed head rolling into view. Queen’s belt is laid down as a trophy beside the gruesome scene.
Ted makes a final approach to Karen, urging her again to abandon the gang and return to a normal life. Karen remains resolute, choosing to stay with Queen and the Man-Eaters as they ride out of Medley toward new towns to terrorize. The gang’s freedom is short-lived, however, as the town’s police — armed with circumstantial evidence rather than solid proof — arrest them for Joe Boy’s murder.
In a rare post-credits moment, the authorities release the gang’s leader due to lack of solid evidence, and Queen Betty Connell rejoins the group as they ride off into the unknown, leaving Ted and the town to face the aftermath of the violence and the question of what comes next.
